Journal: bioRxiv

Article Title: TMIGD1, a putative tumor suppressor, induces G2-M cell cycle checkpoint arrest in colon cancer cells

doi: 10.1101/2020.06.06.138057

Figure Lengend Snippet: ( A ) Two human colorectal cancer tissue microarray slides consist of 172 tissue samples were stained with anti-TMIGD1 antibody. Staining was scored as 0 (negative, <5% cells positive), 1+ (6-25% cells positive), 2+ (26-50% cells positive), and 3+ (>50% cells positive). The tumor differentiation was graded morphologically (grade 1, 2 and 3 as well, moderately and poorly differentiated). ( B ) The average staining intensities were compared using ANOVA with Tukey post-hoc test per tumor grade. ( C ) The average staining intensities were compared using ANOVA with Tukey post-hoc test per tumor differentiation. ( D ) Western blot analysis of TMIGD1 expression in human renal and colorectal cancer cell lines. ( E ) qPCR analysis of TMIGD1 expression in CRC cell lines (HT29, HCT116 and RKO). Human kidney epithelial cells, HK2 was used as a positive control.

Article Snippet: Two human colorectal cancer tissue microarray slides (US Biomax, catalog numbers BC05012a and BC05118a) consist of 172 tissue samples (72 on BC05012a and 100 on BC05118a) were stained with anti-TMIGD1 antibody.

Techniques: Microarray, Staining, Western Blot, Expressing, Positive Control

Journal: Oncotarget

Article Title: CD133 + CD24 lo defines a 5-Fluorouracil-resistant colon cancer stem cell-like phenotype

doi: 10.18632/oncotarget.12168

Figure Lengend Snippet: A. Scheme of genome-wide gene expression analysis. LS411N-CD133 + cells were compared to LS411N-5FU-R cells and SW620-CD133 + cells were compared to SW620-5FU-R cells by DNA microarray analysis. Shown are phenotypes of the two pairs of cells. B. Analysis of the differentially expressed genes between CD133 + cells and 5-FU-R cells. Genes whose expression levels are changed by at least 2 fold (either up-regulated or down-regulated) were selected. The ratios are LS411N-CD133 + /LS411N-5FUR and SW620-CD133 + /SW620-5FU-R. The number of differentially expressed genes of each pair and the commonly differentially expressed genes of the two pairs of cells are shown. C. The commonly differentially expressed genes in two pairs of cells as shown in A and B (n=207) was selected. Cluster 3.0 program was used to analyze the gene expression patterns in a one-dimensional hierarchical clustering to generate gene dendrograms based on the pair-wise calculation of the Pearson coefficient of normalized fluorescence ratios as measurements of similarity and linkage clustering. The clustered data were loaded into TreeView program and displayed by the graded color scheme. Genes that have known functions in stem cell maintenance (CD24, LGR5, and SOX2), cell proliferation, and death (PCNA, CASP3, MLKL, DUSP5, DUSP6, NFKB2, and HDAC5), and for immune response (IL17RB and IL20RB) are indicated. Red columns indicate genes whose expression level is higher in LS411N-CD133 + and SW620-CD133 + cells as compared to LS411N-5FU-R and SW620-5FU-R cells, respectively. Green columns indicate genes whose expression level is higher in LS411N-5FU-R and SW620-5FU-R cells as compared to LS411N-CD133 + and SW620-CD133 + cells, respectively. The color bar at the bottom panel represents the level of differential expression. The number above the bar indicates the fold changes.

Article Snippet: Human Colon cancer tissue microarray slides were provided by CHTN (Mid-Atlantic Division, University of Virginia, Charlottesville, VA).

Techniques: Genome Wide, Expressing, Microarray, Fluorescence